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tet plko neo vector  (Addgene inc)


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    Addgene inc tet plko neo vector
    Tet Plko Neo Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tet plko neo vector/product/Addgene inc
    Average 94 stars, based on 59 article reviews
    tet plko neo vector - by Bioz Stars, 2026-05
    94/100 stars

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    Biological pathways and genes targeted by MSI2. ( A ) Pathway analysis of MSI2-regulated genes for diseases and functions. The analysis was conducted with high-confidence genes showing differential regulation in genes upregulated in the polysome upon MSI2 knockdown. Plotted are the top 20 pathways and corresponding –log 10 ( P -value). ( B ) Western blot for candidate genes CDK6 and EIF3A upon stable <t>lentiviral</t> expression of either control (scramble) shRNA or MSI2 shRNA. Also included are western blots for MSI2 (showing knockdown of MSI2 with shRNA expression) and loading control (β-actin). ( C ) Genome coverage plots of CDK6 and EIF3A corresponding to 3′UTR from iCLIP for MSI2. ( D ) Quantification of EIF3A and CDK6 western blots (compared with loading control) from three replicates (β-actin). * denotes a P -value of 0.033 and ** denote a P -value of 0.008. EIF3A changed by 1.61-fold and CDK6 changed by 1.72-fold from all the replicate gels with β-actin as loading control.
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    Biological pathways and genes targeted by MSI2. ( A ) Pathway analysis of MSI2-regulated genes for diseases and functions. The analysis was conducted with high-confidence genes showing differential regulation in genes upregulated in the polysome upon MSI2 knockdown. Plotted are the top 20 pathways and corresponding –log 10 ( P -value). ( B ) Western blot for candidate genes CDK6 and EIF3A upon stable lentiviral expression of either control (scramble) shRNA or MSI2 shRNA. Also included are western blots for MSI2 (showing knockdown of MSI2 with shRNA expression) and loading control (β-actin). ( C ) Genome coverage plots of CDK6 and EIF3A corresponding to 3′UTR from iCLIP for MSI2. ( D ) Quantification of EIF3A and CDK6 western blots (compared with loading control) from three replicates (β-actin). * denotes a P -value of 0.033 and ** denote a P -value of 0.008. EIF3A changed by 1.61-fold and CDK6 changed by 1.72-fold from all the replicate gels with β-actin as loading control.

    Journal: NAR Cancer

    Article Title: Integrative genome-wide analysis reveals EIF3A as a key downstream regulator of translational repressor protein Musashi 2 (MSI2)

    doi: 10.1093/narcan/zcac015

    Figure Lengend Snippet: Biological pathways and genes targeted by MSI2. ( A ) Pathway analysis of MSI2-regulated genes for diseases and functions. The analysis was conducted with high-confidence genes showing differential regulation in genes upregulated in the polysome upon MSI2 knockdown. Plotted are the top 20 pathways and corresponding –log 10 ( P -value). ( B ) Western blot for candidate genes CDK6 and EIF3A upon stable lentiviral expression of either control (scramble) shRNA or MSI2 shRNA. Also included are western blots for MSI2 (showing knockdown of MSI2 with shRNA expression) and loading control (β-actin). ( C ) Genome coverage plots of CDK6 and EIF3A corresponding to 3′UTR from iCLIP for MSI2. ( D ) Quantification of EIF3A and CDK6 western blots (compared with loading control) from three replicates (β-actin). * denotes a P -value of 0.033 and ** denote a P -value of 0.008. EIF3A changed by 1.61-fold and CDK6 changed by 1.72-fold from all the replicate gels with β-actin as loading control.

    Article Snippet: Lentiviral vector pLKO-Tet-On (Addgene plasmid #21916) was used to generate inducible knockdown clones of MSI2 and EIF3A in the K562 cell line ( ) (details are provided in the Supplementary Methods).

    Techniques: Knockdown, Western Blot, Expressing, Control, shRNA